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Titers: What Do They Tell Us? April 8, 2004 PHChristy: Good evening everyone and thanks for coming! Our topic tonight is "Titers: What do they tell us?" PHChristy: I'll be making some opening comments on this subject. If you have a comment or question, simply type a ? or ! into the room, and wait until you are called on. PHChristy: Please don't chat in the room once I get started. If we have time at the end we can go back to open chat. :) PHChristy: Before I start, let me make it clear that I am NOT a veterinarian or a medical professional. PHChristy: I am a dog breeder, a former medical journalist, and someone who has been involved with holistic care and raising of dogs for over 18 years. PHChristy: I am not here to give medical advice or tell you if or how to use titer tests to make vaccine decisions. I'm simply here to share my thoughts, research, and experience on titers. PHChristy: Only you and your dog's veterinarian can decide how best to protect your dog from disease. PHChristy: OK, let's begin. PHChristy: First, let me explain what a "titer" is. PHChristy: A "titer" can be used to measure how much of a substance is present in a liquid, but in this case, we are talking about how much of an antibody is present in the bloodstream at a particular time. PHChristy: A "titer" measures how many times the sample could be diluted before there was no more antibody found. PHChristy: A 1:2 titer means that the solution could be diluted only twice before no antibody was found. PHChristy: A 1:1200 titer means that the solution could be diluted 1200 times before no antibody was found. PHChristy: The higher the second number, the more antibody was present. PHChristy: Many people who want to minimize vaccines hope to be able to use "titers" to see if their dog needs a "booster shot." PHChristy: Many people will tell them that these tests are not reliable, and encourage them to re-vaccinate instead. PHChristy: What is the truth? Do titer tests tell us anything? PHChristy: The truth is, in nearly all cases, the only thing titer tests might "miss" is not a dog who IS NOT immune, not a dog who IS immune. PHChristy: In other words, if your dog has a protective titer to a virus, you can feel pretty confident that they are, in fact, protected against that virus. PHChristy: (Bacterial immunity is different, as is immunity to some types of virus we don't currently vaccinate against in dogs, so I'm not discussing those here tonight.) PHChristy: Where titer tests are unreliable is that a dog might show a low titer but actually be immune. This is because titer tests don't measure immunity directly. Immunity is a function of cells known as "memory cells," but we cannot measure this. PHChristy: How memory cells work: marcus5495: ? PHChristy: When immune system cells known as "T cells" are exposed to antigens such as viruses, they divide into two groups of cells. One group, antibodies, attacks the invading substance, while the other group becomes memory cells. PHCurious: Marcus, ask your question marcus5495: Okay, so we are talking about antibodies to viruses only. Right? PHChristy: That's right PHChristy: and also, only to the viruses that dogs are vaccinated against. There are some feline viruses, and human viruses, that we vaccinate for that dont' work this way PHChristy: Back to memory cells... :) PHChristy: Dr. Donald Hamilton, a holistic veterinarian and author of "Homeopathic Care for Cats and Dogs: Small Doses for Small Animals," compares antibodies to fire engines. PHChristy: Just because the fire engines aren't racing all over town all the time, and the fire fighters are back in the firehouse, sleeping, eating or playing cards, doesn't mean they aren't ready to jump in their trucks and head to the fire when the alarm sounds. PHChristy: This is why a zero or low titer does not NECESSARILY mean your dog is not immune to a disease. PHChristy: Furthermore, research is showing that memory cells do not actually need reminders to keep working years down the road: PHChristy: "According to Dr. Rafi Ahmed, of the Emory Vaccine Center at Emory University in Atlanta, Georgia, it has always been thought that the memory cells needed reminders. PHChristy: "For a vaccine to offer the most protection against disease, it was believed that it had to refresh the memory of these cells by exposing them to an antigen repeatedly, he explained. PHChristy: "But the current research 'seriously questions that hypothesis,' Ahmed said." PHChristy: The article goes on to explain that two experiments done on mice involving transplanted cells showed "that immune system cells do not have to be reminded to recognize an antigen," and PHChristy: "(I)nstead of focusing on developing immunizations that repeatedly expose the immune system to an antigen, it may be more effective to work on vaccines that produce a strong initial response, since memory cells seem to be able to do their job without being reminded ...." PHChristy: (Citation: Science 1999;286:1377-1381, 1381-1383.) PHChristy: Are there any questions at this point? :) Lesya: kind of PHCurious: Go ahead Lesya Scottie3_nr: I'd like to raise a question I've asked before--does even minimal titer in dicate immunity? herdndog_nr: ! Lesya: So with the studies of Dr. Ahmed, would you suggest that only the initial vaccine would be enough to program the WBC in the immune system to protect against the virus? PHChristy: hang on Scottie.... first is Lesya, then herdndog, and then you PHChristy: Lesya, I'll be getting to that in a few minutes. Great question! Lesya: bt you should use Titers to be absolutely sure? Lesya: Also (sorry) Lesya: I am active in obedience, agility, and Schutzhund PHChristy: I think that NOTHING is "absolutely sure," neither vaccination nor titers Lesya: the club that I do agility abd obedience with require shot records every time you sign up Lesya: how do you suggest going about that? PHCurious: Herd, you will be next PHChristy: but there is more research telling us that titers to viruses correlate with protection against disease, than tell us that repeating a vaccine does PHChristy: Lesya, my suggestion is to educate the clubs and businesses PHChristy: they are not vets PHChristy: they are not medical professionals PHChristy: there is good information out there about duration of immunity to viruses, as well as the possible harm of vaccines Lesya: right. but they're probably erring on the side of caution to protect all dogs and participants. I'll let the others ask their questions PHChristy: and remember, an UNNEEDED vaccines is all risk with no benefit, even if the risk is small PHChristy: I don't agree they are erring on the side of caution Lesya: why is that? PHChristy: they are potentially harming your dog for something that has no scientific basis PHChristy: because if your dog doesn't need that "booster shot," it's all risk, no benefit Lesya: but simply following the "old" beliefs on vaccinations PHChristy: yes, they are Lesya: what are the risks? PHChristy: that's very true PHChristy: Lesya, that's off the subject of titers, so I'll defer that question to another night! Lesya: haha, sorry. no problem PHChristy: but be aware that every veterinary vaccine has a package insert with the known risks and side effects listed right on it PHChristy: OK, next is herdndog, right Curious? herdndog_nr: re: working towards vaccines that would produce a stronger initial response.... please explain why that's different than now. PHCurious: Yes! PHChristy: herdndog, that is actually human research I was quoting. In dogs we are already doing that, in creating things such as the Proguard parvo vaccine that contains much larger amounts of virus PHChristy: they also sometimes include irritants known as "adjuvants" in vaccines (mostly killed virus vaccines) in the hope of stimulating a stronger immune response PHChristy: these things increase the immune response and also increase the risk, especially of potential allergic reactions PHChristy: herdndog, did that answer your question? herdndog_nr: then if you are using the vaccines currently and getting sufficient titers... then why risk a higher virus load? PHChristy: herdndog, I would certainly agree with that herdndog_nr: k PHChristy: Scottie, please go ahead with your question now PHCurious: ! Scottie3_nr: I wonder: Can even a minimal titer indicate immunity? PHChristy: actually... herdndog, let me just quickly add that in the case of parvo, they are doing it to try to overcome maternal antibody interference in puppies PHChristy: Scottie, yes it can Lesya: ? PHChristy: it doesn't always, because some titer tests are not that accurate at lower measurements herdndog_nr: ! PHChristy: but most immunologists will agree that if there ARE antibodies to a normal virus, it generally means you have memory cells PHChristy: and it is my understanding that parvo and distemper in dogs are very accurate in titer tests PHChristy: Scottie, did that cover it for you? Scottie3_nr: Would you, if you had a low titer and did not want to vaccinate, or boooster, have another titer run at a later time? Debbie_nr: Hi everyone...I had SO MUCH trouble logging in here...is there anyway to get a transcript of what has been said so far? PHChristy: Debbie, yes, I will send the transcirpt to the reminder list, the BeyondVaccination list, Wellpet, and K9 Nutrition lists Debbie_nr: TYVM PHChristy: and it will be posted on the "Features" page at www.doghobbyist.com PHChristy: welcome! PHChristy: Scottie, I don't think a titer later on is that helpful, as generally the titer will gradually decline over time Debbie_nr: ? PHChristy: however, there is no reason NOT to do a second titer just in case of lab error MariasMom: ? PHChristy: Curious, go ahead with your comment Scottie3_nr: Are you talking about years? I meant, would a second titer perhaps a few weeks after a low titer result, be advisable before making any vaccination decision? PHCurious: Christy, I've tried to have titers conducted on one human (me) and my cats. They were cost prohitive. PHChristy: Scottie, the titer normally will go down, not up, over a long period of time. A second test in a short period of time would be more to deal with lab error PHCurious: For example, the titer for feline distemper was about $400, while the vaccine is $20 PHChristy: My cats get feline distemper titer for $35 PHChristy: you are being ripped off PHCurious: What's the process like for dog illness titers PHChristy: there is simply no reason on earth to pay anything remotely like that for a titer test PHCurious: ACK!! They told me $100 each for the FVR, C, P, and extra C Scottie3_nr: ! PHChristy: that is INSANE Valkyrie: ! PHChristy: I live in the San Francisco Bay Area, some of the highest vet bills in the nation PHCurious: Oh goodness. OK. That makes a huge difference PHCurious: Lesya, you may ask your question now Lesya: ok PHChristy: Because Valkyrie is a vet tech, I'm going to let her make her comment out of order PHChristy: Val, please ga Valkyrie: I was just appalled by the cost and Valkyrie: there's no reason that any vet should be charging Valkyrie: that sort of price. I would want to know what lab he's using Valkyrie: as well as his stand on vaccines PHCurious: Thanks Val. I will discuss this with my vet, who told me that's what the lab charges her :( Valkyrie: It is known that vets who think titers are bogus Valkyrie: to overcharge their clients. PHChristy: I want to also add that while cost DOES matter, it doesn't matter SCIENTIFICALLY Valkyrie: To convince them to have the vaccine instead. Valkyrie: I know for a fact that the price you were quoted...if using antech is FAR FAR FAR above hospital cost. PHCurious: Well I had a similar thing trying to get myself titered. (I work in hospitals and have to show proof of vax) PHChristy: an unneeded vaccine is still unneeded, regardless of how much the test costs PHChristy: and vice versa PHCurious: Gotcha PHChristy: OK, sorry, let's get back to Lesya's question. Lesya, please ga Lesya: its ok Valkyrie: done. Lesya: I remember in one of my textbooks there was a diagram about dormant viruses and a live virus and it is injected in a mouse. certain reactions, even with the dormant/dead virus caused the subject to be infected with the virus and die. does this ring a bell with anything you've read? Would this apply to the vaccines with our dogs? PHCurious: Thanks Valkyrie! PHChristy: Lesya, it's called reversion to virulence, and while rare, it does happen. PHChristy: RARELY Lesya: I couldn't remember exactly what it was called PHCurious: Herd, your turn! herdndog_nr: whatlevel constitutes "lower measurements on titer test " being inaccurate? Lesya: with revaccination, not only might it be unneccessary, but it will also put your dog in more risk of this to happen, correct? PHChristy: herdndog, the specific number will vary depending on the lab used and the virus in question PHChristy: Lesya, yes Lesya: k PHChristy: although let me stress it's VERY RARE Lesya: yes, I understand PHChristy: most laboratories will indicate the levels they consider protective when they return the results to your vet herdndog_nr: k PHChristy: I discuss this more later PHCurious: Mothergoose, your question? Natalie_nr: I have two dogs, One has had one rabies vac, the other has never been vaccinated. Their titers indicated a higher level of immunity for the non vaccinated dog,can you explain? PHCurious: Natalie, we're doing a structured chat tonight. Mothergoose: Are titers for rabies, and parvovirus, distemper helpful.....or applicable even if you vaccinate? Or can you go only by titers to determine to vaccinate? Also, can you titer for heartworm? PHCurious: I will put you in queue after Scottie Natalie_nr: oh...sorry PHChristy: Mothergoose.... I believe that titers for those three viruses are very reliable and helpful if you are wanting to know if vaccination was successful. I'll discuss this a bit more in a minute. PHChristy: You can look for antibodies to heartworm in the blood, that is what a heartworm test is, but since there is no vaccine for heartworm, it's not really the same as the vaccine issue PHChristy: also, immunity to things like heartworm is very short lived PHChristy: nothing at all like viral immuity Debbie_2_nr: I apologize for sending this question now out of queue...I keep getting bumped off, and I would like to see it in the transcript if I get bumped off again...I am Debbie and Debbie_2. My regular holistic vet, Dr. Susan Wynn, was not in today, and one of our Alaskan Klee Kais was suffereing with vomiting and diarrhea. As a result, she had to see another vet at the clinic (not holistic, of course). I was given the third degree about rabies and how it was illegal not to give the shot. I asked about the titers and could they run the test to see if she even needed the rabies (which I soooooooo don't want to give). I was told that the three-year shot is only good after they've had the initial puppy one (which is only good for a year -- I actually think they're good for nothing). They are running the titers test, but I noticed their language was for her level on "this specific day." My question is how can I legally NOT give the shot? Will a titers letter hold up at a vet clinic? PHChristy: Debbie, there is no state in the US that recognizes titers for rabies in lieu of vaccination at this time. PHChristy: for dogs PHChristy: the CDC reports that no dog or cat who has had two rabies vaccines has ever gotten rabies PHChristy: beyond that, you'll have to discuss this with Susan PHChristy: MOthergoose, did that cover it for you? Scottie3_nr: ! Mothergoose: Yes...but is there an immunity program for Heartworm that is holistic...no ivermectin, etc. PHChristy: Mothergoose, I understand, but that's not related to titers PHChristy: titers measure SPECIFIC immunity, which is the process of antibodies and memory cells. Those approaches boost GENERAL immunity, hoping that the immune system will handle the heartworm if it occurs Mothergoose: Okay...thanks. PHCurious: Debbie, has your question been answered? Debbie_2_nr: Curious...yes, thanks! PHChristy: I'd like to move on, so if we can just hold the rest of the questions fo ra few more minutes.... PHCurious: Great PHChristy: Curious, please keep the queue until I'm done with the last section PHCurious: OK, next will be Scottie, then Natalie. PHCurious: If anyone else would like to be added, please type ! or ? PHChristy: OK, I'm going to go on.... PHChristy: Many people argue that there is no "proof" that titers are related to immunity, but that's not true. PHChristy: Besides their very common use in human medicine, research has also been done in veterinary medicine. PHChristy: One such study was done by Michael R. Lappin DVM PhD DACVIM, a leading veterinary virology researcher. In a 2002 study, Dr. Lappin found that the feline panluekopenia (similar to canine parvovirus) and feline calicivirus antibody correlation with protection when challenged with the diseases were .... drumroll .... 100 percent. Debbie_2_nr: ? PHChristy: THIS WAS TRUE EVEN IN CATS WHO HAD ANTIBODY LEVELS LOWER THAN THOSE CONSIDERED "PROTECTIVE." PHChristy: (Citation: Lappin MR, Andrews J, Simpson D, et al. "Use of serologic tests to predict resistance to feline herpesvirus 1, feline calcivirus, and feline parvovirus infection in cats." Journal of the American Veterinary Medical Assocation 2002.) PHChristy: What this means in your decision making process is that if you use titers to tell you when to vaccinate, you will be far more likely to end up needlessly vaccinating some animals that don't need it, not missing animals who DO need it. PHChristy: This is the opposite conclusion than what most people who object to titers for being "inaccurate" seem to have reached! PHChristy: One last thought. While it's true that titers aren't well understood or tested, neither are animal vaccines. Mothergoose: ? PHChristy: Length of duration studies and long term safety studies are virtually never done and are not required to approve an animal vaccine. PHChristy: I find it very troubling that so many people criticize titer tests for their supposed "unreliability," while acting as if the act of giving a "booster shot" has been proven to increase immunity - which in the case of the canine viruses parvo and distemper has in fact been proven NOT to be true. PHChristy: You cannot make an immune dog "more immune," and the antibodies from the vaccines given to the dog in the past will simply wipe out the virus in the new vaccine, and no "booster" effect takes place. You'll thus have all the risk, and no benefit. PHChristy: (Citation: Schultz, R.D., "Current and Future Canine and Feline Vaccination Programs." Vet Med 3: No. 3, 233-254, 1998.) PHChristy: Hopefully things are changing, as more and more pet owners are asking for titer tests and questioning the science behind vaccine protocols. PHChristy: I'll now open this up to any remaining comments or questions. Thanks, everyone! PHChristy: and I think that means it's Scottie's turn, LOL PHCurious: Scottie, you're up! PHCurious: Now that I know how much they REALLY cost, I'll be asking for titers! PHChristy: :D Scottie3_nr: I understand that there is new "in office" titering equipment that cuts the cost dramatically. Lesya: ? PHChristy: Scottie, yes, that's true. I'm not sure how effective it is compared to sending it out to the lab, as it's fairly new. I'll be watching this with great interest. PHChristy: But it's an example of the kind of things we'll see as more owners ask for titer tests PHChristy: prices will come down PHCurious: Natalie, you have a question or comment? PHChristy: and more kinds of tests will be widely available Natalie_nr: I have two dogs. One vaccinated for rabies one time, Natalie_nr: the other never vaccinated. I had them titered. The non vaccinated dog had a higher level of immunity than the vaccinated dog, Can you explain? PHCurious: Prices better come down at my vet's office... PHChristy: Natalie, this is for rabies, right? Natalie_nr: yes PHChristy: Natalie, that does happen from time to time and it always baffles people, because rabies is a killed virus vaccine PHChristy: killed virus supposedly does not replicate, and therefore there shouldn't be any "shedding" of the vaccine virus PHChristy: in the case of other vaccines, the modified live virus replicates in the dog's body and is shed in the stool, and other dogs in the house or the park or whatever can get exposed to it and develop a titer that way PHChristy: but with rabies it really is very much inexplicable PHChristy: either the virus IS shedding PHChristy: or the other dogs are somehow getting exposed to the virus and not getting sick, which I find EXTREMELY hard to believe PHCurious: ? I'm putting myself in queue :) PHChristy: but you are not the only person I've known who has experienced this Natalie_nr: Thanks...I was curious as to how it could occur PHChristy: me too! PHChristy: I'm sure in the coming years we'll find the answer PHCurious: Curiosity is good PHChristy: LOL Curious PHChristy: ok, who's next? :) PHCurious: Debbie, you're up! Debbie_2_nr: Another question: My other AKK developed fear aggression after his first puppy rabies shot. I was told by the vet assistant today that the rabies shot would have had nothing to do with that. I disagreed. What are your thoughts? Can I get a letter stating that he cannot have another rabies shot due to fear aggression? PHChristy: The second question will depend on your vet. Many vets would give you an exemption letter based on this, and many wouldn't. Scottie3_nr: 1 Debbie_2_nr: gotta find the right vet, eh?! Debbie_2_nr: thanks Scottie3_nr: ! PHChristy: The first question, my thoughts are that any virus that grows in the cells of the nervous system could obviously have the potential to cause behavior problems PHChristy: and I have heard FAR too many reports of this to dismiss it PHChristy: there are other possible explanations, but I don't believe the rabies vaccine can be ruled out as a cause based on what we know today PHChristy: ga PHCurious: Mothergoose, your turn Debbie_2_nr: I guess that's because you are brilliant AND open-minded...what a combination! Thanks so much! PHChristy: LOL Debbie, wow, thank you so much!!! PHChristy: you've certainly made MY night! Laurie_nr: ? Debbie_2_nr: :-) Mothergoose: When is the dog too old to vaccinate? PHChristy: Mothergoose, I can't answer that question. To me, the issue is always one of risks vs benefits. PHChristy: There is no set age at which you can say, the risk will always outweigh the benefits PHChristy: but I can tell you, EVERY dog is "too old" for an unneccessary vaccine PHChristy: or too young, or too sick, etc PHChristy: you have to look at the RISK of the disease and the RISK of the vaccine, and make an informed decision. And also do your best to determine if the vaccine is needed, ie, is the dog already immune from previous vaccines or illness
PHCurious: Lesya, give her a tough one!
Lesya: what about the set of vaccines a puppy gets? would you say that the repeated vaccination within the short span of time is harmful or necessary for initiating (can't spit the right word out) immunity?
PHChristy: Lesya, puppies don't need a series of vaccines to become immune. What they need is ONE VACCINE THAT WORKS.
Lesya: so just one initial vaccine
Lesya: then titers after that
PHChristy: You can give a vaccine and then two weeks later do a titer test, and know if the vaccine worked. In other words, if immunity formed.
PHChristy: No, just ONE THAT WORKS
PHChristy: a titer test will tell you if it worked
Lesya: ok
PHChristy: once you have that post-vaccine titer response, there is no scientific reason to repeat the vaccination
PHCurious: Lesya, did that answer your question?
PHChristy: if you give a vaccine and do NOT have a post-vaccine titer response, that puppy is not immunized even if they are vaccinated
Lesya: so there are vaccines that are duds?
PHChristy: Lesya, yes, but usually it's maternal antibody interference, not a problem with the vaccine itself
Lesya: ok.
PHChristy: especially with parvo
Lesya: ill have to check my vet for titers
MariasMom: !
PHChristy: and parvo maternal antibody can persist until the puppy is nearly 6 months old
PHChristy: go ahead with the next question or comment
PHCurious: You're up Scottie
Scottie3_nr: Re: "Natural immujnity" to rabies--wasn't the rabies vaccine originally MLV? Could such a virus remain viable in, say, soil, for a long period?
PHChristy: I think MariasMom will have to be last as we are almost out of time
PHCurious: OK
PHChristy: Scottie, yes, and some MLV has been given to wildlife. But I don't believe it's stable in soil AT ALL, it requires an animal vector
PHChristy: next?
PHCurious: Laurie, you're up
Laurie_nr: my bitch has an autoimmune disease. does that compromise a titer?
PHChristy: Laurie, what it does is contraindicate vaccination! Vaccines are licensed for use in healthy, clinically normal animals only. Furthermore, autoimmune disease is one of the known side effects of vaccines in dogs.
PHChristy: But as far as I know, no, it doesn't compromise the accuracy of a titer test.
Laurie_nr: thanks
PHChristy: ga
PHCurious: OK, last question of the evening is from Mariasmom
MariasMom: once you have that post-vaccine titer showing it "worked" there is no reason to continue titering year after year in "lieu" of vaccination, right?
PHChristy: MariasMom, in my opinion, none, except curiousity
PHCurious: WHAT??
PHChristy: but other people prefer to do more tests, it is sort of a crutch
PHChristy: Curious, you cannot "boost" immunity
PHCurious: My vet told me every year x$500 per cat...
PHChristy: you can't make an animal more immune to a virus
PHCurious: Oh, I've got phone calls to make
MariasMom: I hear so many say they are going to titer every year instead of vaccinating
PHChristy: Curious.... you need a new vet
PHChristy: Hi Lin!!! Chat is just ending!
Valkyrie: !
PHChristy: Folks, Small Dog Chat will be starting in just a moment
PHChristy: Val, please ga
LINANATOR: I figured I was too late, but I tried. :)
Natalie_nr: goodnight thank you Christie
Valkyrie: Unfortunately, there are boarding facilities that fancy themselves veterinary medicine savvy. They may be the reason some people have to titer every year.
Debbie_2_nr: tyvm Christy for your time and knowledge-sharing
PHCurious: Thank you Christie! Helping with this chat just saved me thousands of dollars :)
PHChristy: What I would personally dio, and this is just my personal opinion.... is titer after intitial vaccination to make sure it "worked," and then I'd be done.
Scottie3_nr: TYVM
PHChristy: Speaking about VIRUSES
PHCurious: Val, oh this is true. One once told me titer tests were only good if taken within 2 weeks of boarding
Lesya: and the antibodies stay active for the rest of the dog's life?
PHChristy: There is a real lack of understanding of how the immune system works out there!
PHChristy: Lesya, not the antibodies, the memory cells
PHChristy: as I said earlier
Lesya: ...same thing! lol....
PHChristy: although for many dogs and cats, also the antibodies remain
Lesya: im joking
PHChristy: LOL
Valkyrie: That's crazy.
Lesya: how soon after vaccination shouuld one titer?
Valkyrie: And yet so many will accept a bordatella vaccine the same day.
PHChristy: Two weeks after
Lesya: k
PHChristy: it takes up to two weeks for immunity to form after a vaccine is given
PHChristy: Val, that's true! which is just nuts
PHCurious: OK dog folks, I need to run. I'm a cat host, but it was fun being here with you all tonight. And good for my checking account too. :)
PHCurious: Thank you Christie and pups
Lesya: bye curious
PHChristy: Goodnight Curious, thank you so much!
Valkyrie: Boarding kennels and petstore owners that practice veterinary medicine are one of my pet peeves. LOL
Lesya: nice to meet you!
Valkyrie: G'night
PHCurious: Goodnight everyone!
PHChristy: Hi there DunMadra!
DunMadra: Hey Christy
Lesya: thanks christy for gracing us wth your oh so knowledgable presence
Lesya: ;-)
PHChristy: We are just wrapping up our special chat on Titers
PHChristy: LOL Lesya, thank YOU!
PHChristy: Thanks to everyone for coming tonight!
Lesya: I've been leaning awy from the DVM
DunMadra: christy ohhh sorry I missed the titer chat
Lesya: and this is very interesting
Lesya: might get into the Biology & Virology
PHChristy: Lesya, a fascinating field!
Lesya: but I'm not s ure if I couuld handle the lab setting
PHChristy: Dun, I will post the link to the transcript
PHChristy: it was a great chat
Lesya: nor testing on animals
DunMadra: thanks
Valkyrie: Yes it was. A very good chat.
Lesya: and I don't think being an animal behaviorist makes enough $$ to support the critters or myself
Lesya: very informative
Lesya: I could feel my brain swelling with info :E
PHChristy: LOL
Lesya: this is the stuff that gets my heart pumping
Lesya: calculus... pfffsha
PHChristy: harv, I'll post the link to the transcript to the reminder list, on the "Features" page at www.doghobbyist.com, and to the K9 Nutrition, BeyondVaccination, and Wellpet lists
Valkyrie: I've been reading Diodati's book. Informative.
PHChristy: Val, I just got it
PHChristy: I've been meaning to read it since it came out
Lesya: what's the book on?
PHChristy: it's on cat and dog vaccination
Valkyrie: Vaccinations
Lesya: I'm a book nerd
Lesya: title / author that I could find it under?
PHChristy: I actually don't think that anyone is here for small dog chat!
PHChristy: author is Catherine Diodati
Valkyrie: Diodati
DunMadra: ohhh christy...the can of worms is open now PHChristy: not sure of the exact title
PHChristy: LOL DunMadra
Valkyrie: What every pet owner should know about vaccinations I believe.
PHChristy: OK, Ginger is here and I have to run because... guess why, LOL
DunMadra: Howdy Ginger
PHChristy: and I also need to eat and feed my dogs
PHGinger: Hello everyone
Lesya: yup
Valkyrie: See ya later.
Lesya: ive gotta walk my girl
Valkyrie: Hi Ginger
PHChristy: I'll see you all next week, THANK YOU for a great chat!
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